H9c2 2-1 cells
WebWhen cultured in low-serum containing medium, H9c2 myoblasts exit the cell cycle and differentiate into myocytes/myotubes. A key step of this process is the expression of … WebCell line name: H9c2(2-1) Synonyms: H9c2 (2-1); H9c2; H9C2: Accession: CVCL_0286: Resource Identification Initiative: To cite this cell line use: H9c2(2-1) (RRID:CVCL_0286) …
H9c2 2-1 cells
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WebH9c2 (2-1) cells were cultured for 1, 2, 3 days, the proliferation of cells were assayed by cell counting Kit-8 assay, the expressions of GLUT4 mRNA and protein were detected … WebEfficiency of DNA transfection of rat heart myoblast cells H9c2(2-1) by either polyethyleneimine or electroporation. Expression of exogenous DNA in vitro is …
WebH9C2 (2-1) Cell Line from rat. BDIX heart myoblast, 88092904. All Photos (1) Synonym(s): H9c2 Cells. Recommended Products. Slide 1 of 10. 1 of 10. Sigma-Aldrich. 306-05A. …
WebFeb 21, 2024 · Briefly, H9c2 cells were incubated with ZYZ-772 at different concentrations for 2 h before possessing to the 1 mM CoCl 2. After 12 h the medium was removed from the wells, added 100 uL MTT (0.5 mg/mL) to each well, and the samples were then incubated for 4 h at 37 °C. WebPurchase high quality cryopreserved rat BDIX heart myoblast H9c2 cells from ECACC for myoblast differentiation, transfection, cardiomyocyte and muscle biology. US EN. …
WebH9C2 cells would continue to take in Ca2+, decreasing extra-mitochondrial Ca2+levels. Figure 2: The images on the left are the un-differentiated and differentiated H9C2 cells. After differentiation, the cardiac myoblasts turned into “cardiac myocyte-like” cells. Due to mutations that could have occurred, it is hard to tell their exact
WebFeb 1, 2015 · 2.1. Cell cultures. H9c2 embryonic rat heart-derived (ventricular) cells (myoblasts) from ATCC were cultured in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% fetal bovine serum (FBS) under 95% air/5% CO 2, and subcultured when at 50–60% confluence. Notably, a higher degree of confluence was associated … inhaling formalinWebMar 1, 2016 · And the population doubling time for H9C2 is 2.3 days if it grows well. So if I want it to achieve confluency at different day, I seed the cells according to split ratio as follows: 1: 2... mkdir : no such file or directory phpWebFeb 8, 2024 · The pcDNA3.1-MALAT1, MALAT1 siRNA (si-MALAT1) and their corresponding controls were purchased from Shanghai GenePharma Co., Ltd. H9C2 cells (2×105cells/ml) were transfected at 37°C for 24 h with Lipofectamine®2000 (Invitrogen; Thermo Fisher Scientific, Inc.) as previously described (20). mkdirsync modeWebCell information for H9c2(2-1). Find diseases associated with this biological target and compounds tested against it in bioassay experiments. inhaling freon gasWebMar 22, 2024 · ( d) H9c2 cells were cultured in retinoic acid (RA) and 1% FBS for 5 days to induce differentiation and maturation. Differentiated H9c2 cells were then cultured with KL (200 ng/mL), FGF23 (100 ng/mL), KL (200 ng/mL) plus FGF23 (100 ng/mL), KL (200 ng/mL) + anti-FGF23 (1 μg/mL), or KL (200 ng/mL) plus anti-KL (1 μg/mL) for 3 days. inhaling fumesWebWhat is the recommended seeding density for H9C2 cells (specifically in a plastic tc t25 flask)? We have been seeding at the recommended 10x10^4/cm^2 (250,000 in t25) and … mkdir syscallWebBased on our proteomic analysis, its presence in EVs was linked to hypoxia in HL1 and H9c2 cells, and we hypothesize that EV-associated ENPL may have a cardioprotective … mkdir /var/cache/nginx/client_temp failed